Assessment of Toxicity, Genotoxicity, and Cytotoxicity of Phenycycloalkanpolycarboxylic Acids: A Study with Allium cepa and Chlorella vulgaris

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Abstract

To assess the toxicity, cytotoxicity, and genotoxicity of polycarbonic acids containing cycloaliphatic fragments, a comprehensive approach was used, based on the application of test organisms (the unicellular green alga Chlorella vulgaris and onion Allium cepa). The polycarbonic acid compounds were evaluated at concentrations of 1.0, 0.1, 0.001, and 0.0001%. It was shown that the polyacids were not mutagenic in the Allium cepa based bioassay, but high concentrations of the compounds could lead to an increase in the mutation frequency of Chlorella vulgaris. These results justify the need for further research to comprehensively evaluate the safety and potential applicability of these compounds. The approach described here can be applied to obtain quick, cost-effective, and useful supplementary data on various types of toxicity in vitro for substances with potential biological activity.

About the authors

A. A Firstova

Federal State Budgetary Educational Institution of Higher Education “Yaroslavl State Technical University”

Email: firstova.a.a@mail.ru
Yaroslavl, Russia

M. I Kovaleva

Federal State Budgetary Educational Institution of Higher Education “Yaroslavl State University named after P.G. Demidova”

Yaroslavl, Russia

E. R Kofanov

Federal State Budgetary Educational Institution of Higher Education “Yaroslavl State Technical University”

Yaroslavl, Russia

M. V Tarasenko

Center for Transfer of Pharmaceutical Technologies named after M.V. Dorogov, a structural subdivision of the Federal State Budgetary Educational Institution of Higher Education “Yaroslavl State Pedagogical University named after K.D. Ushinsky”

Yaroslavl, Russia

References

  1. Cordonni A., Gomez-Catalan J., Jimenez A.I., Cativiela C., Perez J.J. // J. Peptide Sci. 2002. V. 8. P. 253–266. https://doi.org/10.1002/psc.383
  2. Christensen H.N., Handlogten M.E., Lam I., Tager H.S., Zand R. // J. Biol. Chem. 1969. V. 244. P. 1510–1520. https://doi.org/10.1016/S0021-9258(18)91789-8
  3. van Winkle L.J., Christensen H.N., Campione A.L. // J. Biol. Chem. 1985. V. 260. P. 118–123.
  4. Mo F, Li J., Yan Y, Wu W, Lai S. // Drug Des. Devel. Ther. 2018. V. 12. P. 3583–3594. https://doi.org/10.2147/DDDTS166544
  5. Wiviott S.D., Steg P.G. // Lancet. 2015. V. 386. P. 292–302. https://doi.org/10.1016/S0140-6736(15)60213-6
  6. Luna M., Holper E.M. // Curr. Atheroscler. Rep. 2015. V. 17. P. 483–484. https://doi.org/10.1007/s11883-014-0483-4
  7. Capranzano P., Capodanno D. // Expert Rev. Cardiovasc. Ther. 2013. V. 11. P. 307–317. https://doi.org/10.1586/erc.13.3
  8. Duerschmied D., Ahrens I., Mauler M., Brandt C., Weidner S., Bode C., Moser M. // PLOS One. 2012. V. 7. P. e32656. https://doi.org/10.1371/journal.pone.0032656
  9. Floyd C.N., Ferro A. // Prostaglandins Other Lipid Mediat. 2015. V. 120. P. 21–27. https://doi.org/10.1016/j.prostaglandins.2015.03.011
  10. Czopek A., Kubacka M., Bucki A., Siwek A., Filipek B., Pawlowski M., Kolaczkowski M. // Pharmacol. Rep. 2021. V. 73. P. 1361–1372. https://doi.org/10.1007/s43440-021-00284-6
  11. Kubacka M., Kazek G., Kotanska M., Filipek B., Waszkielewicz A.M., Mogilski S. // Eur. J. Pharmacol. 2018. V. 818. P. 263–270. https://doi.org/10.1016/j.ejphar.2017.10.053
  12. Chu X.J., Bartkovitz D., Danho W., Swistok J., Cheung A.W., Kurylko G., Rowan K., Yeon M., Franco L., Qi L., Chen L., Yagaloff K. // Bioorg. Med. Chem. Lett. 2005. V. 15. P. 4910–4914. https://doi.org/10.1016/j.bmcl.2005.08.012
  13. Fan W., Boston B.A., Kesterson R.A., Hruby V.J., Cone R.D. // Nature. 1997. V. 385. P. 165–168. https://doi.org/10.1038/385165a0
  14. Huszar D., Lynch C.A., Fairchild-Huntress V., Dunmore J.H., Fang Q., Berkemeier L.R., Gu W., Kesterson R.A., Boston B.A., Cone R.D., Smith F.J., Campfield L.A., Burn P., Lee F. // Cell. 1997. V. 88. P. 131–141. https://doi.org/10.1016/s0092-8674(00)81865-6
  15. Burdick D.J., Skelton N.J., Ultsch M., Beresini M.H., Eigenbrot C., Li W., Zhang Y., Nguyen H., KongBeltran M., Quinn J.G., Kirchhofer D. // ACS Chem. Biol. 2020. V. 15. P. 425–436. https://doi.org/10.1021/acschembio.9b00899
  16. Garg G., Forsberg L.K., Zhao H., Blagg B.S. // Chemistry. 2017. V. 23. P. 16574–16585. https://doi.org/10.1002/chem.201703206
  17. Solit D.B., Chiosis G. // Drug Discov. Today. 2008. V. 13. P. 38–43. https://doi.org/10.1016/j.drudis.2007.10.007
  18. Neckers L., Workman P. // Clin. Cancer Res. 2012. V. 18. P. 64–76. https://doi.org/10.1158/1078-0432.CCR-11-1000
  19. Gary G., Zhao H., Blagg B.S. // Bioorg. Med. Chem. 2017. V. 25. P. 451–457. https://doi.org/10.1016/j.bmc.2016.11.030
  20. Sunden H., Ma J.N., Hansen L.K., Gustavsson A.L., Burstein E.S., Olsson R. // ChemMedChem. 2013. V. 8. P. 1283–1294. https://doi.org/10.1002/cmdc.201300175
  21. Shao L., Hewitt M.C., Wang F., Malcolm S.C., Ma J., Campbell J.E., Campbell U.C., Engel S.R., Spicer N.A., Hardy L.W., Schreiber R., Spear K.L., Varney M.A. // Bioorg. Med. Chem. Lett. 2011. V. 21. P. 1434–1437. https://doi.org/10.1016/j.bmcl.2011.01.019
  22. Cases S., Smith S.J., Zheng Y.W., Myers H.M., Lear S.R., Sande E., Novak S., Collins C., Welch C.B., Lusis A.J., Farese R.V.Jr. // Proc. Natl. Acad. Sci. USA. 1998. V. 95. P. 13018–13023. https://doi.org/10.1073/pnas.95.22.13018
  23. Bakir W.A., Gaidan H.A., Al-Kaabi M.M. // Indian J. Pathol. Microbiol. 2020. V. 63. P. 230–234. https://doi.org/10.4103/IPM.IIPM_460_19
  24. Firstova A.A., Kofanov E.R. // Russ. J. Org. Chem. 2023. V. 59. P. 820–825. https://doi.org/10.1134/S1070428023050123
  25. Pat. Ru 2790910 CI Method for obtaining 4-carboxy phenyleycloalkanedicarboxylic acids / Firstova A.A., Kofanov E.R. // Federal State Yaroslavl Educational Institution of Higher Education “Yaroslavl State Technical University”.
  26. Firstova A.A., Kofanov E.R., Krasovskaya G.G., Danilova A.S. // Russ. Chem. Bull. 2017. V. 66. P. 867–869. https://doi.org/10.1007/s11172-017-1820-x
  27. Prokhorova I.M. // Biol. Inland Waters. 2008. V. 2. P. 17–23.
  28. Firstova A.A., Kofanov E.R., Kovaleva M.I. // Russ. J. Bioorg. Chem. 2023. V. 49. P. 65–75. https://doi.org/10.1134/S1068162023010089

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