Psychrotolerant Strains of the Genus Trichoderma from Bottom Sediments of Lake Baikal as Promising Biopesticides’ Producers

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Abstract

Among 54 psychrotolerant strains of 16 species of the genus Trichoderma isolated from the bottom grounds of Lake Baikal 12 strains were selected during sequential screening on solid and liquid media. Finally, 6 strains of the species Trichoderma atroviride, T. gamsii, T. harzianum and Trichoderma sp. were selected. They exhibited inhibitory action towards test-microorganisms Aspergillus niger INA 00760, Bacillus subtilis ATCC6633, Candida albicans ATCC2091 or Escherichia coli ATCC25922 as well as towards strains of phytopathogens Pectobacterium carotovorum VKM-B1247 and Fusarium oxysporum VKPM F-148. Maximum antibiotic activity was established for the culture fluid (CF) and CF concentrate of Trichoderma sp. 2d-4B strain during submerged cultivation against Pectobacterium carotovorum VKM-B1247 and Fusarium oxysporum VKPM F-148. Based on the data of the effective suppression of these phytopathogens and on an assessment of the effect of this strain on the germination of seeds of the monocotyledonous plant Triticum aestivum and the dicotyledonous plant Cucumis sativus and on the length of their sprouts it was shown that the strain Trichoderma sp. 2d-4B can be used to create a biopreparation.

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About the authors

Yu. A. Roshka

Gause Institute of New Antibiotics

Author for correspondence.
Email: roshkajulia@gmail.com
Russian Federation, Moscow

A. V. Kurakov

Moscow State University

Email: kurakov57@mail.ru

кафедра микологии и альгологии, Биологический факультет

Russian Federation, Moscow

M. D. Fedorova

Moscow State University

Email: kurakov57@mail.ru

кафедра микологии и альгологии, Биологический факультет

Russian Federation, Moscow

V. S. Sadykova

Gause Institute of New Antibiotics

Email: sadykova_09@mail.ru
Russian Federation, Moscow

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2. Fig. 1. Seeds of cucumber Cucumis sativus (variety Garmonist F1, Agrofirma Gavrish) and soft wheat Triticum aestivum after soaking in the test solutions: A and B – cucumber seeds on the first and third days after soaking, respectively; B – wheat seeds on the third day after soaking.

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